Primary Identifier | MGI:3029503 | Allele Type | Targeted |
Attribute String | Null/knockout, Reporter | Gene | Foxl2 |
Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The sequence encoding amino acids 62-375 was replaced with an in frame nls-lacZ cassette followed by a floxed neomycin resistance gene. This deletes almost the entire the forkhead/winged-helix domain. The neomycin gene was subsequently excised. Western blot analysis of protein extracts from neonatal ovaries detected only lacZ fusion proteins and not the wild-type protein. |