| Primary Identifier | MGI:3032598 | Allele Type | Targeted |
| Attribute String | Humanized sequence | Gene | Trp53 |
| Transmission | Germline | Strain of Origin | 129 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | An arginine to proline substitution was generated at codon 172 by an engineered point mutation introduced at coding nucleotide 515 (c.515G>C). p.R172P, equivalent to p.R175P in humans, is associated with human tumors defective in apoptosis. A single loxP site was left in intron 4 after the excision of a loxP site flanked neomycin resistance gene cassette via Cre-mediated recombination. Western blot analysis of homozygous MEFs detected full length protein at levels that exceeded those of full length protein in wild-type MEFs. |
