| First Author | Ordureau A | Year | 2013 |
| Journal | J Biol Chem | Volume | 288 |
| Issue | 34 | Pages | 24569-80 |
| PubMed ID | 23846693 | Mgi Jnum | J:203446 |
| Mgi Id | MGI:5527035 | Doi | 10.1074/jbc.M113.479550 |
| Citation | Ordureau A, et al. (2013) DEAF1 is a Pellino1-interacting protein required for interferon production by Sendai virus and double-stranded RNA. J Biol Chem 288(34):24569-80 |
| abstractText | Double-stranded (ds) RNA of viral origin, a ligand for Melanoma Differentiation-associated gene 5 (MDA5) and Toll-Like Receptor 3 (TLR3), induces the TANK-Binding Kinase 1 (TBK1)-dependent phosphorylation and activation of Interferon Regulatory Factor 3 (IRF3) and the E3 ubiquitin ligase Pellino1, which are required for interferon beta (IFNbeta) gene transcription. Here, we report that Pellino1 interacts with the transcription factor Deformed Epidermal Autoregulatory Factor 1 (DEAF1). The interaction is independent of the E3 ligase activity of Pellino1, but weakened by the phosphorylation of Pellino1. We show that DEAF1 binds to the IFNbeta promoter and to IRF3 and IRF7, that it is required for the transcription of the IFNbeta gene and IFNbeta secretion in MEFs infected with Sendai virus or transfected with poly(I:C). DEAF1 is also needed for TLR3-dependent IFNbeta production. Taken together, our results identify DEAF1 as a novel component of the signal transduction network by which dsRNA of viral origin stimulates IFNbeta production. |
