Primary Identifier | MGI:3034030 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Endog |
Transmission | Germline | Strain of Origin | 129 |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The majority of the 3 exons of the Endog and 5 of 10 exons of D2Wsu81e (which overlaps exon 3 of Endog) were replaced with a neomycin selection cassette inserted by homologous recombination. Reduced ENDOG protein levels were detected by Western blot analysis of protein extracts obtained from heterozygous MEFs and hearts. |