| First Author | Vandenbroucke St Amant E | Year | 2012 |
| Journal | Circ Res | Volume | 111 |
| Issue | 6 | Pages | 739-49 |
| PubMed ID | 22798526 | Mgi Jnum | J:212645 |
| Mgi Id | MGI:5581914 | Doi | 10.1161/CIRCRESAHA.112.269654 |
| Citation | Vandenbroucke St Amant E, et al. (2012) PKCalpha activation of p120-catenin serine 879 phospho-switch disassembles VE-cadherin junctions and disrupts vascular integrity. Circ Res 111(6):739-49 |
| abstractText | RATIONALE: Adherens junctions (AJs) are the primary intercellular junctions in microvessels responsible for endothelial barrier function. Homophilic adhesion of vascular endothelial (VE) cadherin forms AJs, which are stabilized by binding of p120-catenin (p120). p120 dissociation from VE-cadherin results in loss of VE-cadherin homotypic interaction and AJ disassembly; however, the signaling mechanisms regulating p120 dissociation from VE-cadherin are not understood. OBJECTIVE: To address the mechanism of protein kinase C (PKC)-alpha function in increasing endothelial permeability, we determined the role of PKCalpha phosphorylation of p120 in mediating disruption of AJ integrity. METHODS AND RESULTS: We showed that PKCalpha phosphorylation of p120 at serine (S)879 in response to thrombin or lipopolysaccharide challenge reduced p120 binding affinity for VE-cadherin and mediated AJ disassembly secondary to VE-cadherin internalization. In studies in mouse lung vessels, expression of the phosphodeficient S879A-p120 mutant prevented the increase in vascular permeability induced by activation of the thrombin receptor PAR-1. CONCLUSIONS: PKCalpha phosphorylation of p120 at S879 is a critical phospho-switch mediating disassociation of p120 from VE-cadherin that results in AJ disassembly. Therefore, blocking PKCalpha-mediated p120 phosphorylation represents a novel targeted anti-inflammatory strategy to prevent disruption of vascular endothelial barrier function. |
