| First Author | Harigae H | Year | 1998 |
| Journal | Genes Cells | Volume | 3 |
| Issue | 1 | Pages | 39-50 |
| PubMed ID | 9581981 | Mgi Jnum | J:115103 |
| Mgi Id | MGI:3690673 | Doi | 10.1046/j.1365-2443.1998.00166.x |
| Citation | Harigae H, et al. (1998) Differential roles of GATA-1 and GATA-2 in growth and differentiation of mast cells. Genes Cells 3(1):39-50 |
| abstractText | BACKGROUND: While mast cells have been previously shown to express both GATA-1 and GATA-2 mRNAs, individual functions for these related factors during their course of differentiation within the mast cell lineage have not yet been defined. To address this question, the expression of GATA-1 and GATA-2 mRNAs and proteins were examined in three mouse mast cell progenitor lines as well as in mast cells isolated from both wild-type and GATA-1-deficient mice. RESULTS: Both mast cell progenitor lines, as well as primary mouse bone marrow-derived mast cells (BMMCs) and peritoneal mast cells (PMCs) were examined by RNA blotting and immunological analyses. GATA-2 protein was abundantly expressed in all three mast cell lines and in BMMCs, but only weakly in some of PMCs. In contrast, GATA-1 protein was expressed in PMCs and BMMCs after culture in the presence of IL3 and SCF. We also found the presence of Alcian blue staining-positive but berberine staining-negative mast cells in the skin of mice heterozygous to GATA-1 knock-down allele. CONCLUSION: These results suggest that the expression of GATA factor-dependent genes is regulated by GATA-2 during mast cell development and that GATA-1 is required for the specification of differentiated mast cell phenotypes. |
