| First Author | Qiu R | Year | 2008 |
| Journal | J Cell Biol | Volume | 181 |
| Issue | 6 | Pages | 973-83 |
| PubMed ID | 18541704 | Mgi Jnum | J:137223 |
| Mgi Id | MGI:3798348 | Doi | 10.1083/jcb.200708091 |
| Citation | Qiu R, et al. (2008) Regulation of neural progenitor cell state by ephrin-B. J Cell Biol 181(6):973-83 |
| abstractText | Maintaining a balance between self-renewal and differentiation in neural progenitor cells during development is important to ensure that correct numbers of neural cells are generated. We report that the ephrin-B-PDZ-RGS3 signaling pathway functions to regulate this balance in the developing mammalian cerebral cortex. During cortical neurogenesis, expression of ephrin-B1 and PDZ-RGS3 is specifically seen in progenitor cells and is turned off at the onset of neuronal differentiation. Persistent expression of ephrin-B1 and PDZ-RGS3 prevents differentiation of neural progenitor cells. Blocking RGS-mediated ephrin-B1 signaling in progenitor cells through RNA interference or expression of dominant-negative mutants results in differentiation. Genetic knockout of ephrin-B1 causes early cell cycle exit and leads to a concomitant loss of neural progenitor cells. Our results indicate that ephrin-B function is critical for the maintenance of the neural progenitor cell state and that this role of ephrin-B is mediated by PDZ-RGS3, likely via interacting with the noncanonical G protein signaling pathway, which is essential in neural progenitor asymmetrical cell division. |
