| First Author | Feng JQ | Year | 2003 |
| Journal | J Dent Res | Volume | 82 |
| Issue | 10 | Pages | 776-80 |
| PubMed ID | 14514755 | Mgi Jnum | J:85859 |
| Mgi Id | MGI:2677174 | Doi | 10.1177/154405910308201003 |
| Citation | Feng JQ, et al. (2003) The Dentin Matrix Protein 1 (Dmp1) is Specifically Expressed in Mineralized, but not Soft, Tissues during Development. J Dent Res 82(10):776-80 |
| abstractText | Dentin Matrix Protein 1 (Dmp1) was originally identified from dentin. However, its expression and function in vivo are not clear. To clarify these two issues, we have generated mice carrying a truncated Dmp1 gene by using gene targeting to replace exon 6 with a lacZ gene. Northern blot analysis shows the expected 5.8-kb Dmp1-lacZ fusion transcript and loss of the wild-type 2.8-kb Dmp1 transcript, confirmed by a lack of immunostaining for the protein. Using heterozygous animals, we demonstrate that Dmp1 is specific for mineralized tissues. Not previously shown, Dmp1 is also expressed in pulp cells. Dmp1-deficient embryos and newborns display no apparent gross abnormal phenotype, although there are a modest expansion of the hypertrophic chondrocyte zone and a modest increase in the long bone diameter. This suggests that DMP1 is not essential for early mouse skeletal or dental development. |
