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Publication : Applying real-time monitoring of circadian oscillations in adult mouse brain slices to study communications between brain regions.

First Author  Huang S Year  2021
Journal  STAR Protoc Volume  2
Issue  2 Pages  100416
PubMed ID  33870223 Mgi Jnum  J:358573
Mgi Id  MGI:6728156 Doi  10.1016/j.xpro.2021.100416
Citation  Huang S, et al. (2021) Applying real-time monitoring of circadian oscillations in adult mouse brain slices to study communications between brain regions. STAR Protoc 2(2):100416
abstractText  This protocol combines a protective cutting method to prepare various brain slices from adult mice and real-time monitoring of circadian oscillations to measure circadian rhythmicity in various brain slices. This protocol can be applied to studies of how brain damages affect local circadian clocks and subsequent circadian variations in nearby areas. Further functional analyses with in vivo systems will determine whether these circadian variations are detrimental or beneficial to the brain. For complete details on the use and execution of this protocol, please refer to Huang et al. (2020).
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