| First Author | Hirano A | Year | 2014 |
| Journal | Mol Cell Biol | Volume | 34 |
| Issue | 24 | Pages | 4464-73 |
| PubMed ID | 25288642 | Mgi Jnum | J:224319 |
| Mgi Id | MGI:5662019 | Doi | 10.1128/MCB.00711-14 |
| Citation | Hirano A, et al. (2014) In vivo role of phosphorylation of cryptochrome 2 in the mouse circadian clock. Mol Cell Biol 34(24):4464-73 |
| abstractText | The circadian clock is finely regulated by posttranslational modifications of clock components. Mouse CRY2, a critical player in the mammalian clock, is phosphorylated at Ser557 for proteasome-mediated degradation, but its in vivo role in circadian organization was not revealed. Here, we generated CRY2(S557A) mutant mice, in which Ser557 phosphorylation is specifically abolished. The mutation lengthened free-running periods of the behavioral rhythms and PER2::LUC bioluminescence rhythms of cultured liver. In livers from mutant mice, the nuclear CRY2 level was elevated, with enhanced PER2 nuclear occupancy and suppression of E-box-regulated genes. Thus, Ser557 phosphorylation-dependent regulation of CRY2 is essential for proper clock oscillation in vivo. |
