| Primary Identifier | MGI:3043876 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Cd274 |
| Transmission | Germline | Strain of Origin | 129S/SvEv |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exon 1, encoding the start codon and signal peptide, and a portion of exon 2, encoding the IgV domain, were replaced with a neo cassette inserted by homologous recombination. Protein was undetected on the surface of homozygous mutant CD4+ T cells via flow cytometric analysis. Western blot analysis further confirmed the absence of normal protein in homozygous mutant mice. |
