| Primary Identifier | MGI:3045169 | Allele Type | Targeted |
| Attribute String | Humanized sequence, Inserted expressed sequence | Gene | Col2a1 |
| Transmission | Germline | Strain of Origin | 129S4/SvJae-Tg(Prm-cre)70Og |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A 3xHA-tagged human SOX9 cDNA sequence and floxed neo were inserted upstream of exon 2 by homologous recombination. Transient cre-mediated recombination excised the neo, leaving only the human SOX9 insertion sequence. Northern blot of rib cartilage in heterozygous mutant mice revealed knocked-in SOX9 gene expression was ~20% of that of endogenous Sox9 gene expression. |
