| First Author | Lancien M | Year | 2021 |
| Journal | J Immunol | Volume | 207 |
| Issue | 2 | Pages | 421-435 |
| PubMed ID | 34233909 | Mgi Jnum | J:315063 |
| Mgi Id | MGI:6727809 | Doi | 10.4049/jimmunol.2000498 |
| Citation | Lancien M, et al. (2021) Dendritic Cells Require TMEM176A/B Ion Channels for Optimal MHC Class II Antigen Presentation to Naive CD4(+) T Cells. J Immunol |
| abstractText | Intracellular ion fluxes emerge as critical actors of immunoregulation but still remain poorly explored. In this study, we investigated the role of the redundant cation channels TMEM176A and TMEM176B (TMEM176A/B) in retinoic acid-related orphan receptor gammat(+) cells and conventional dendritic cells (DCs) using germline and conditional double knockout mice. Although Tmem176a/b appeared surprisingly dispensable for the protective function of Th17 and group 3 innate lymphoid cells in the intestinal mucosa, we found that they were required in conventional DCs for optimal Ag processing and presentation to CD4(+) T cells. Using a real-time imaging method, we show that TMEM176A/B accumulate in dynamic post-Golgi vesicles preferentially linked to the late endolysosomal system and strongly colocalize with HLA-DM. Taken together, our results suggest that TMEM176A/B ion channels play a direct role in the MHC class II compartment of DCs for the fine regulation of Ag presentation and naive CD4(+) T cell priming. |
