| First Author | Ferron M | Year | 2005 |
| Journal | Genesis | Volume | 41 |
| Issue | 3 | Pages | 138-45 |
| PubMed ID | 15754380 | Mgi Jnum | J:97319 |
| Mgi Id | MGI:3575220 | Doi | 10.1002/gene.20108 |
| Citation | Ferron M, et al. (2005) Targeted expression of Cre recombinase in macrophages and osteoclasts in transgenic mice. Genesis 41(3):138-45 |
| abstractText | To develop specific conditional gene ablation in the hematopoietic myeloid-osteoclast lineage, transgenic mice expressing Cre recombinase under the control of the CD11b promotor were generated on the C57BL/6 background. The cellular specificity of Cre activity following recombination was quantified in the Z/EG reporter transgenic mice by FACS analysis with lineage-specific markers and EGFP coexpression. A high degree of recombination, as evidenced by EGFP-positive cells, was demonstrated in macrophages and granulocytes of bone marrow and spleen by the presence of double-positive cells CD11b/EGFP and Gr1/EGFP, respectively. Interestingly, the peritoneal macrophage population showed almost complete DNA recombination at large. Most important, mature osteoclast cells derived from the double transgenic bone marrow and spleen progenitors were EGFP-positive. Hence, these CD11b-Cre mice will provide a unique tool to unravel novel gene function and activities involved during osteoclast and macrophage differentiation and maturation processes. |
