| Primary Identifier | MGI:3045792 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Rfx3 |
| Transmission | Germline | Strain of Origin | 129/Sv |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Cre-mediated recombination of Rfx3tm1Wrth resulted in the excision of floxed exon 3, leaving an FRT flanked neo. Exon 3 encodes the DNA binding domain. RT-PCR showed that the deletion of exon 3 resulted in the splicing of exon 2 to exon 4, leading to a frameshift and premature termination at an out-of-frame stop codon. |
