| Primary Identifier | MGI:3045799 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Sfrp1 |
| Transmission | Germline | Strain of Origin | 129S5/SvEvBrd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exon 1, which encodes most of the protein and the entire CRD, was replaced by a LacZ/MC1-neo cassette. PCR analysis demonstrated that homozygous mutants contained the LacZ gene. Northern blot of kidneys indicated no presence of transcript for the CRD domain of the endogenous gene. RT-PCR analysis of calvaria showed lack of expression of both the CRD (the 5' region of the transcript) and the netrin domain (the 3' region) in mutants. |
