| Primary Identifier | MGI:3047632 | Allele Type | Targeted |
| Attribute String | Inserted expressed sequence | Gene | Pax3 |
| Transmission | Germline | Strain of Origin | 129S2/SvPas |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Crossing of Pax3tm3(Pax7)Buck with mice expressing Cre in the germline create this allele in which the DsRed-FRT-puro-pA was excised, allowing expression of knocked in Pax7. In situ hybridization showed that Pax3 transcripts are detectable in mutant embryos, suggesting either the use of a cryptic promoter and/or alternative splicing, or leakiness of the 3' poly A sequence following the nLacZ. Immuno histochemistry indicates binding of antibodies to beta-gal in somites of mutants. |
