Primary Identifier | MGI:3047789 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Xist |
Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
Is Recombinase | false | Is Wild Type | false |
molecularNote | In vitro cre expression excised the floxed PGK-neomycin resistance gene in Xisttm3Nbd, leaving the transcriptional terminator site, SPA-MAZ4 in the region 1.1 kb upstream of the P1 start site. RT-PCR indicates presence of ectopic sense transcription upstream of Xist in mutants. |