| First Author | Haas T | Year | 2017 |
| Journal | Eur J Immunol | Volume | 47 |
| Issue | 5 | Pages | 872-879 |
| PubMed ID | 28295265 | Mgi Jnum | J:247184 |
| Mgi Id | MGI:5923272 | Doi | 10.1002/eji.201646775 |
| Citation | Haas T, et al. (2017) Card9 controls Dectin-1-induced T-cell cytotoxicity and tumor growth in mice. Eur J Immunol 47(5):872-879 |
| abstractText | Activation of the C-type lectin receptor Dectin-1 by beta-glucans triggers multiple signals within DCs that result in activation of innate immunity. While these mechanisms can potently prime CD8+ cytotoxic T-cell (CTL) responses without additional adjuvants, the Dectin-1 effector pathways that control CTL induction remain unclear. Here we demonstrate that Dectin-1-induced CTL cross-priming in mice does not require inflammasome activation but strictly depends on the adapter protein Card9 in vitro. In vivo, Dectin-1-mediated Card9 activation after vaccination drives both expansion and activation of Ag-specific CTLs, resulting in long-lasting CTL responses that are sufficient to protect mice from tumor challenge. This Dectin-1-induced antitumor immune response was independent of NK cell function and completely abrogated in Card9-deficient mice. Thus, our results demonstrate that Dectin-1-triggered Card9 signaling but not inflammasome activation can potently cross-prime Ag-specific CTLs, suggesting that this pathway would be a candidate for immunotherapy and vaccine development. |
