| First Author | Badiani P | Year | 1994 |
| Journal | Genes Dev | Volume | 8 |
| Issue | 7 | Pages | 770-82 |
| PubMed ID | 7926766 | Mgi Jnum | J:126456 |
| Mgi Id | MGI:3761365 | Doi | 10.1101/gad.8.7.770 |
| Citation | Badiani P, et al. (1994) Dominant interfering alleles define a role for c-Myb in T-cell development. Genes Dev 8(7):770-82 |
| abstractText | The transcription activator c-Myb is expressed at high levels in immature thymocytes and during T-cell activation and may be a regulator of T-cell differentiation. To investigate the role of c-Myb in T-cell development, we generated transgenic mice in which two dominant interfering Myb alleles, one a competitive inhibitor of DNA binding, and the other, an active repressor comprising the Myb DNA-binding domain linked to the Drosophila Engrailed transcription repressor domain, were expressed from early times onward in T cells. Both alleles partially blocked thymopoiesis and inhibited proliferation of mature T cells. The Myb-En chimera was the more efficient repressor and might serve as an archetype for the manufacture of other dominant interfering transcription factor alleles. |
