| First Author | Karandikar SH | Year | 2019 |
| Journal | JCI Insight | Volume | 5 |
| PubMed ID | 30869653 | Mgi Jnum | J:290288 |
| Mgi Id | MGI:6442334 | Doi | 10.1172/jci.insight.127882 |
| Citation | Karandikar SH, et al. (2019) New epitopes in ovalbumin provide insights for cancer neoepitopes. JCI Insight 5 |
| abstractText | MHC I-restricted epitopes of chicken ovalbumin (OVA) were originally identified using CD8 T cells as probes. Here, using bioinformatics tools, we identify four additional epitopes in OVA in addition to a cryptic epitope. Each new epitope is presented in vivo, as deduced from the lack of CD8 response to it in OVA-transgenic mice. In addition, CD8 responses to the known and novel epitopes are examined in C57BL/6 mice exposed to the OVA-expressing tumor E.G7 in several ways. No responses to any epitope including SIINFEKL are detected in mice with growing E.G7 or mice immunized with the tumor. Only in E.G7-bearing mice treated with an anti-CTLA4 antibody which depletes tumor-infiltrating regulatory T cells, CD8 responses to SIINFEKL and the novel epitope EKYNLTSVL are detected. Finally, all epitopes fails to treat mice with pre-existing tumors. These observations force an important re-consideration of the common assumptions about the therapeutic value of neoepitopes detected by CD8 responses in tumor-bearing hosts. |
