| First Author | Schultz JR | Year | 1992 |
| Journal | J Biol Chem | Volume | 267 |
| Issue | 30 | Pages | 21630-6 |
| PubMed ID | 1400473 | Mgi Jnum | J:92450 |
| Mgi Id | MGI:3052645 | Doi | 10.1016/S0021-9258(19)36658-X |
| Citation | Schultz JR, et al. (1992) Expression of human apolipoprotein A-II and its effect on high density lipoproteins in transgenic mice. J Biol Chem 267(30):21630-6 |
| abstractText | Apolipoproteins A-I and A-II comprise approximately 70 and 20%, respectively, of the total protein content of HDL. Evidence suggests that apoA-I plays a central role in determining the structure and plasma concentration of HDL, while the role of apoA-II is uncertain. To help define the function of apoA-II and determine what effect increasing its plasma concentration has on HDL, transgenic mice expressing human apoA-II and both human apoA-I and human apoA-II were produced. Human apoA-II mRNA is expressed exclusively in the livers of transgenic animals, and the protein exists as a dimer as it does in humans. High level expression of human apoA-II did not increase HDL concentrations or decrease plasma concentrations of murine apoA-I and apoA-II in contrast to what was observed in mice overexpressing human apoA-I. The primary effect of overexpressing human apoA-II was the appearance of small HDL particles composed exclusively of human apoA-II. HDL from mice transgenic for both human apoA-I and human apoA-II displayed a unique size distribution when compared with either apoA-I or apoA-II transgenic mice and contain particles with both these human apolipoproteins. These results in mice, indicating that human apoA-II participates in determining HDL size, parallel results from human studies. |
