| Primary Identifier | MGI:3052158 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Ezr |
| Transmission | Germline | Strain of Origin | 129S4/SvJae |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | LoxP sites were inserted to flank exons 3-5. An FRT-flanked promoterless Beta-galactosidase-neomycin cassette was inserted into intron 5. Crossing to EIIa-Cre mice excised the floxed region containing exons 3-5 and left the FRT-flanked Beta-geo. Immunohistochemical analysis of mutants confirmed absence of endogenous protein. Western blot of mutant neonate intenstinal tissue also demonstrated absence of protein. |
