| Primary Identifier | MGI:3052727 | Allele Type | Targeted |
| Attribute String | Null/knockout, Recombinase, Reporter | Gene | Epor |
| Transmission | Germline | Strain of Origin | BALB/cJ |
| Is Recombinase | true | Is Wild Type | false |
| molecularNote | An EGFP/icre sequence was inserted into exon 1, replacing the endogenous ATG with the translational start site of the EGFP/icre, yet preserving the last 10 bp of the splice-donor site of exon 1. A FRT flanked neo was inserted downstream to replace parts of exon 2 and exons 3-8. Transient FLP expression excised the neo. Southern blot and multiplex PCR confirmed recombination in heterozygous mice. Immunoblot of FLCs demonstrated green fluorescence in heterozygous and homozygous embryos, with homozygotes showing a 2-fold increase in fluorescence intensity compared to heterozygotes. |
