| Primary Identifier | MGI:3053044 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(TcrAND)53Hed |
| Strain of Origin | (C57BL/6 x SJL)F2 | Is Recombinase | false |
| Is Wild Type | false |
| description | The transgenic TCR is expressed on virtually all T cells in mice expressing Ek. Transgenic mice on a background that involves C57BL/10, C57BL/6, and SJL show expression of a rearranged T-cell receptor specific for the carboxy-terminal fragment of pigeon cytochrome c and the Ek molecule, resulting in a major subpopulation of T cells restricted to class II MHC proteins. These mice have an abnormally high percentage of mature CD4+CD8- cells, a peripheral T-cell population is almost exclusively CD4+ and show no expression of I-Ek. |
| molecularNote | Rearranged TCR alpha and beta genes containing endogenous promoter and enhancer sequences were coinjected into the micronucleus of 1 cell embryos. Both constructs are specific for the carboxyterminal fragment of pigeon cytochrome c and the Ek molecule. The alpha-chain of this transgenic TCR differs by one amino acid (an alanine instead of a threonine at position 99) to the alpha chain of the transgenic TCR expressed in Tg(TcraTcrbAD10)1Hed transgenic mice. |
