Primary Identifier | MGI:3052866 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Tbxas1 |
Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Sequence encoding amino acids 234-333 and the first 233 bp of intron 9 were replaced with an HPRT cassette. Southern blot and PCR using tail DNA confirmed recombination. Northern blot and RT-PCR showed lack of wild-type transcript in spleen and thymus tissues of mutants. |