| Primary Identifier | MGI:3052922 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Mbnl1 |
| Transmission | Germline | Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP-flanked neomycin selection cassette replaced exon 3 and flanking sequence. The neomycin cassette was removed by transient Cre expression in ES cells prior to the production of chimeric mice. RT-PCR analysis demonstrated that no transcripts with sequences from exon 3 were present, but other splice isoforms expressed from this gene were detectable. Immunoblot analysis further confirmed the absence of protein isoforms containing sequences encoded by exon 3. |
