Primary Identifier | MGI:3052750 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Klre1 |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | An EGFP-neo was inserted in frame 9 bp downstream of ATG. Southern blot and RT-PCR indicated recombination was successful and no wild-type transcript was present in mutant splenocytes. Flow cytometry showed lack of surface expression of the endogenous protein. GFP expression inversely correlated with the loss of Klre1 expression. |