| First Author | Ewald D | Year | 1996 |
| Journal | Science | Volume | 273 |
| Issue | 5280 | Pages | 1384-6 |
| PubMed ID | 8703072 | Mgi Jnum | J:35417 |
| Mgi Id | MGI:82866 | Doi | 10.1126/science.273.5280.1384 |
| Citation | Ewald D, et al. (1996) Time-sensitive reversal of hyperplasia in transgenic mice expressing SV40 T antigen. Science 273(5280):1384-6 |
| abstractText | The role of viral oncoprotein expression in the maintenance of cellular transformation was examined as a function of time through controlled expression of simian virus 40 T antigen (TAg). Expression of TAg in the submandibular gland of transgenic mice from the time of birth induced cellular transformation and extensive ductal hyperplasia by 4 months of age. The hyperplasia was reversed when TAg expression was silenced for 3 weeks. When TAg expression was silenced after 7 months, however, the hyperplasia persisted even though TAg was absent. Although the polyploidy of ductal cells could be reversed at 4 months of age, cells at 7 months of age remained polyploid even in the absence of TAg. These results support a model of time-dependent multistep tumorigenesis, in which virally transformed cells eventually lose their dependence on the viral oncoprotein for maintenance of the transformed state. |
