| First Author | Pedrotti LP | Year | 2016 |
| Journal | Eur J Immunol | Volume | 46 |
| Issue | 1 | Pages | 70-80 |
| PubMed ID | 26464149 | Mgi Jnum | J:233858 |
| Mgi Id | MGI:5788226 | Doi | 10.1002/eji.201545585 |
| Citation | Pedrotti LP, et al. (2016) Systemic IL-12 burst expands intestinal T-lymphocyte subsets bearing the alpha(4) beta(7) integrin in mice. Eur J Immunol 46(1):70-80 |
| abstractText | The intestinal immune system is complex and displays unique anatomic and functional characteristics. Numerous immune cell subsets are located beneath the epithelial barrier and their activity is highly regulated. Using hydrodynamic shear of IL-12 cDNA to achieve systemic expression of IL-12 in mice, we evaluated the effect of a transient burst of this cytokine on the activation status of T cells from Peyer's patches (PPs), mesenteric lymph nodes (MLNs), and colonic lamina propria (LP). Following systemic IL-12 release, intestinal T lymphocytes became activated, exhibiting a CD44(high) CD62L(-) phenotype. After 5 days of the cytokine burst, the frequency of alpha4beta7(+) CD4(+) and CD8(+) cells increased, and CD8(+) alpha4beta7(+) cells mainly expressed T bet, a critical regulator of the Th1 differentiation program. The incremental increase in alpha4beta7 expression involved the IL-12 receptor-signal transducer and activator of transcription (STAT)-4 axis, and occurred independently of IFN-gamma, IL-4, IL-10, and TNF-alpha signaling. Moreover, IL-12 priming exacerbated the outcome of acute dextran sodium sulphate (DSS)-induced colitis with higher scores of weight loss, blood in stool, and diarrhea and lower hematocrit. Together, our findings demonstrate that systemic polarizing signals could effectively expand the number of effector cells able to home to the LP and contribute to local inflammation. |
