| First Author | Yokoyama T | Year | 2007 |
| Journal | Arch Virol | Volume | 152 |
| Issue | 3 | Pages | 603-9 |
| PubMed ID | 17106625 | Mgi Jnum | J:278321 |
| Mgi Id | MGI:6323307 | Doi | 10.1007/s00705-006-0858-0 |
| Citation | Yokoyama T, et al. (2007) Both host prion protein 131-188 subregion and prion strain characteristics regulate glycoform of PrP Sc. Arch Virol 152(3):603-9 |
| abstractText | Prion proteins (PrPs) contain 2 N-linked glycosylation sites and are present in cells in 3 different forms. An abnormal isoform of prion protein (PrP(Sc)) has different glycoform patterns for different prion strains. However, the molecular basis of the strain-specific glycoform variability in prions has remained elusive. To understand the molecular basis of these glycoform differences, we analyzed PrP(Sc) in 2 lines of transgenic mice (MHM2 and MH2M with PrP null background) that expressed a chimeric PrP. Our result indicated that PrP 131-188 (substitutions at I139M, Y155N, and S170N) contributed to both PrP(C) and PrP(Sc) glycoform ratios. Furthermore, the PrP(Sc) glycoform pattern within these transgenic mice showed a subtle difference depending on the inoculated prion. This study indicated that the PrP(Sc) glycoform ratio was influenced by both host PrP(C) and the prion strain. |
