| Primary Identifier | MGI:3055340 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Myf5 |
| Transmission | Germline | Strain of Origin | 129S2/SvPas |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A targeting vector was designed to insert a cassette containing EGFP-pA-loxP-puro-loxP into exon 1, thereby deleting the 122 amino acids encoding the basic helix-loop-helix domain. Additionally, the two ATG codons were mutated to GTG. The EGFP contains a Val164Ala mutation in pEGFP-C1. |