| Primary Identifier | MGI:3055889 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Mapk1 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| description | ES cell line EB3/5 is a mutant E14TG2a line carrying one inactivated allele of Pou5f1. Rescue of placental defects by complementation with tetraploid embryos extends survival up to at least E13.5. These rescued mutant mice have a normal heart, exhibit normal placental development and grow as well as wild-type embryos. |
| molecularNote | A targeting vector was used to insert a floxed region containing a splice acceptor, IRES, neo and SV40 pA into intron 2. Mapk1 cDNA with a polyA tail was inserted in place of exon 3. Protein was undetectable by immunoblot analysis. |
