Primary Identifier | MGI:3056344 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Rad17 |
Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A neo cassette was inserted into intron 2 in antisense orientation and a 110 bp segment of exon 3 was deleted resulting in a frameshift and formation of 3 stop codons via homologous recombination. RT-PCR showed the presence of two shorter transcripts and immunoblot results confirmed the absence of protein. |