Primary Identifier | MGI:3056345 | Allele Type | Targeted |
Attribute String | Conditional ready, No functional change | Gene | Nodal |
Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A floxed hygromycin cassette was inserted in intron 1 and a third loxP site was placed in intron 3. In vitro cre recombination removed the floxed hygromycin cassette from properly targeted ES cell clones. Exons 2 and 3 were then left flanked by loxP sites. Mice were crossed to a protamine deleter strain to confirm that the recombined allele had a null phenotype. |