| Primary Identifier | MGI:3055993 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Fosl1 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd-Hprt1<b-m3> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Crossing Fosl1tm2Wag with mice expressing Cre in the embryo proper created this allele in which the floxed region containing exons 3 and 4 (containing the dimerization and the DNA-binding domains) was excised. Upon excision, the 3' GFP was spliced to the N-terminal part of the transcript and was thereby activated. Western blot detected the fusion protein using an anti-GFP antibody. Immunofluorescence confirmed GFP activation. |