| First Author | Bilodeau M | Year | 2014 |
| Journal | Stem Cell Reports | Volume | 3 |
| Issue | 4 | Pages | 634-49 |
| PubMed ID | 25358791 | Mgi Jnum | J:276488 |
| Mgi Id | MGI:6316453 | Doi | 10.1016/j.stemcr.2014.07.010 |
| Citation | Bilodeau M, et al. (2014) Identification of a proximal progenitor population from murine fetal lungs with clonogenic and multilineage differentiation potential. Stem Cell Reports 3(4):634-49 |
| abstractText | Lung development-associated diseases are major causes of morbidity and lethality in preterm infants and children. Access to the lung progenitor/stem cell populations controlling pulmonary development during embryogenesis and early postnatal years is essential to understand the molecular basis of such diseases. Using a Nkx2-1(mCherry) reporter mouse, we have identified and captured Nkx2-1-expressing lung progenitor cells from the proximal lung epithelium during fetal development. These cells formed clonal spheres in semisolid culture that could be maintained in vitro and demonstrated self-renewal and expansion capabilities over multiple passages. In-vitro-derived Nkx2-1-expressing clonal spheres differentiated into a polarized epithelium comprised of multiple cell lineages, including basal and secretory cells, that could repopulate decellularized lung scaffolds. Nkx2-1 expression thus defines a fetal lung epithelial progenitor cell population that can be used as a model system to study pulmonary development and associated pediatric diseases. |
