| First Author | Quwailid MM | Year | 2004 |
| Journal | Mamm Genome | Volume | 15 |
| Issue | 8 | Pages | 585-91 |
| PubMed ID | 15457338 | Mgi Jnum | J:93069 |
| Mgi Id | MGI:3055684 | Doi | 10.1007/s00335-004-2379-z |
| Citation | Quwailid MM, et al. (2004) A gene-driven ENU-based approach to generating an allelic series in any gene. Mamm Genome 15(8):585-91 |
| abstractText | N-ethyl-N-nitrosourea (ENU) introduces mutations throughout the mouse genome at relatively high efficiency. Successful high-throughput phenotype screens have been reported and alternative screens using sequence-based approaches have been proposed. For the purpose of generating an allelic series in selected genes by a sequence-based approach, we have constructed an archive of over 4000 DNA samples from individual F1 ENU-mutagenized mice paralleled by frozen sperm samples. Together with our previously reported archive, the total size now exceeds 6000 individuals. A gene-based screen of 27.4 Mbp of DNA, carried out using denaturing high-performance liquid chromatography (DHPLC), found a mutation rate of 1 in 1.01 Mbp of which 1 in 1.82 Mbp were potentially functional. Screening of whole or selected regions of genes on subsets of the archive has allowed us to identify 15 new alleles from 9 genes out of 15 tested. This is a powerful adjunct to conventional mutagenesis strategies and has the advantage of generating a variety of alleles with potentially different phenotypic outcomes that facilitate the investigation of gene function. It is now available to academic collaborators as a community resource. |
