| First Author | Lobe CG | Year | 1999 |
| Journal | Dev Biol | Volume | 208 |
| Issue | 2 | Pages | 281-92 |
| PubMed ID | 10191045 | Mgi Jnum | J:93700 |
| Mgi Id | MGI:3505578 | Doi | 10.1006/dbio.1999.9209 |
| Citation | Lobe CG, et al. (1999) Z/AP, a double reporter for cre-mediated recombination. Dev Biol 208(2):281-92 |
| abstractText | The Cre/loxP site-specific recombination system combined with embryonic stem cell-mediated technologies has greatly expanded our capability to address normal and disease development in mammals using genetic approaches. The success of this emerging technology hinges on the production of Cre-expressing transgenic lines that provide cell type-, tissue-, or developmental stage-specific recombination between loxP sites placed in the genome. Here we describe and characterize the production of a double-reporter mouse line that provides a convenient and reliable readout of Cre recombinase activity. Throughout all embryonic and adult stages, the transgenic animal expresses the lacZ reporter gene before Cre-mediated excision occurs. Cre excision, however, removes the lacZ gene, allowing expression of the second reporter, the human alkaline phosphatase gene. This double-reporter transgenic line is able to indicate the occurrence of Cre excision in an extremely widespread manner from early embryonic to adult lineages. It will be a valuable reagent for the increasing number of investigators taking advantage of the powerful tools provided by the Cre/loxP site-specific recombinase system. |
