| First Author | Walgrave H | Year | 2023 |
| Journal | iScience | Volume | 26 |
| Issue | 6 | Pages | 106829 |
| PubMed ID | 37250784 | Mgi Jnum | J:351232 |
| Mgi Id | MGI:7487563 | Doi | 10.1016/j.isci.2023.106829 |
| Citation | Walgrave H, et al. (2023) microRNA-132 regulates gene expression programs involved in microglial homeostasis. iScience 26(6):106829 |
| abstractText | microRNA-132 (miR-132), a known neuronal regulator, is one of the most robustly downregulated microRNAs (miRNAs) in the brain of Alzheimer's disease (AD) patients. Increasing miR-132 in AD mouse brain ameliorates amyloid and Tau pathologies, and also restores adult hippocampal neurogenesis and memory deficits. However, the functional pleiotropy of miRNAs requires in-depth analysis of the effects of miR-132 supplementation before it can be moved forward for AD therapy. We employ here miR-132 loss- and gain-of-function approaches using single-cell transcriptomics, proteomics, and in silico AGO-CLIP datasets to identify molecular pathways targeted by miR-132 in mouse hippocampus. We find that miR-132 modulation significantly affects the transition of microglia from a disease-associated to a homeostatic cell state. We confirm the regulatory role of miR-132 in shifting microglial cell states using human microglial cultures derived from induced pluripotent stem cells. |
