| First Author | Kallies A | Year | 2004 |
| Journal | J Exp Med | Volume | 200 |
| Issue | 8 | Pages | 967-77 |
| PubMed ID | 15492122 | Mgi Jnum | J:93944 |
| Mgi Id | MGI:3510293 | Doi | 10.1084/jem.20040973 |
| Citation | Kallies A, et al. (2004) Plasma cell ontogeny defined by quantitative changes in blimp-1 expression. J Exp Med 200(8):967-77 |
| abstractText | Plasma cells comprise a population of terminally differentiated B cells that are dependent on the transcriptional regulator B lymphocyte--induced maturation protein 1 (Blimp-1) for their development. We have introduced a gfp reporter into the Blimp-1 locus and shown that heterozygous mice express the green fluorescent protein in all antibody-secreting cells (ASCs) in vivo and in vitro. In vitro, these cells display considerable heterogeneity in surface phenotype, immunoglobulin secretion rate, and Blimp-1 expression levels. Importantly, analysis of in vivo ASCs induced by immunization reveals a developmental pathway in which increasing levels of Blimp-1 expression define developmental stages of plasma cell differentiation that have many phenotypic and molecular correlates. Thus, maturation from transient plasmablast to long-lived ASCs in bone marrow is predicated on quantitative increases in Blimp-1 expression. |
