| First Author | Chang HS | Year | 2004 |
| Journal | Am J Pathol | Volume | 165 |
| Issue | 5 | Pages | 1535-41 |
| PubMed ID | 15509524 | Mgi Jnum | J:93624 |
| Mgi Id | MGI:3487304 | Doi | 10.1016/S0002-9440(10)63411-6 |
| Citation | Chang HS, et al. (2004) Using siRNA technique to generate transgenic animals with spatiotemporal and conditional gene knockdown. Am J Pathol 165(5):1535-41 |
| abstractText | Based on the RNAi technique, we have developed a new approach that generates transgenic animals capable of mimicking human genetic diseases. The new system is a combination of siRNA with Cre-loxP and tetracycline-on. It has the characteristics of being stable, inheritable, and inducible, with the siRNA able to be transcribed tissue specifically. To support the ability of this new method to generate a model for a disease, we created an ABCA1-deficient mouse line that mimics Tangier disease under controlled conditions. Thus, it should now be possible to rapidly establish human genetic diseases as a whole animal model without the use of embryonic stem cell and gene targeting. This system also provides a tool for pathological and pharmacological studies of aspects peculiar to particular human genetic diseases. |
