| First Author | Dierssen M | Year | 1997 |
| Journal | Brain Res | Volume | 749 |
| Issue | 2 | Pages | 238-44 |
| PubMed ID | 9138724 | Mgi Jnum | J:39408 |
| Mgi Id | MGI:86790 | Doi | 10.1016/s0006-8993(96)01173-0 |
| Citation | Dierssen M, et al. (1997) Alterations of central noradrenergic transmission in Ts65Dn mouse, a model for Down syndrome. Brain Res 749(2):238-44 |
| abstractText | Mice with segmental trisomy 16 (Ts65Dn) which have triplication of a region of mouse chromosome 16 homologous to the Down syndrome critical region in human chromosome 21, are used as a model for Down syndrome. Functioning of the central beta-noradrenergic transmission was studied in Ts65Dn mice. Binding analysis in cerebral cortex revealed no change in the number of beta-adrenoceptors and a slight reduction of affinity. The beta-adrenoceptor transduction was assessed by analyzing cAMP formation in the cerebral cortex, hippocampus and cerebellar cortex under basal conditions and after stimulation with isoprenaline and forskolin. Basal production of cAMP was significantly reduced in hippocampus and cerebellar cortex of Ts65Dn mice compared to control, but not in cerebellum. After phosphodiesterase inhibition, net increments in cAMP accumulation were similar in both groups of mice, Stimulation of cAMP production by isoprenaline (10 mu M) and forskolin (10 mu M) was much higher in hippocampus than in cerebral cortex of either group. In both areas, but not in cerebellum, the stimulatory responses were consistently and significantly smaller in Ts65Dn than in control mice. Concentration-response curves for isoprenaline and forskolin were, generated in the cerebral cortex. E(max) responses were lower in trisomic than in control mice; however, in Ts65Dn mice the slope of the response curve to isoprenaline was markedly depressed whereas that to forskolin was similar to control. It is concluded that Ts65Dn mice show seven deficiencies in the synaptic transmission of the central beta-noradrenergic system, which are selective for specific brain areas. (C) 1997 Elsevier Science B.V. |
