| Primary Identifier | MGI:3511287 | Allele Type | Targeted |
| Attribute String | Not Specified | Gene | Chrna4 |
| Transmission | Germline | Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A point mutation was introduced to change a leucine to alanine within the putative pore-forming M2 domain. A loxP flanked neo cassette was also inserted in the construct. Correctly targeted ES cells were transfected with Cre plasmid to remove the neo. |