| Primary Identifier | MGI:3512111 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Tpst2 |
| Transmission | Germline | Strain of Origin | 129S7/SvEvBrd-Hprt1<b-m2> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A 0.88 kb fragment spanning the start codon and the remainder of exon 3 was replaced by a 1.7 kb PGKneo cassette. A 4.5 kb EcoRI-ApaI genomic fragment was used as the left arm for homologous recombination and was cloned into the pPGKneo vector upstream of the PGKneo cassette. Northern blot analysis and QPCR confirmed the absence of transcription. |
