| Primary Identifier | MGI:3521803 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Slc46a2 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A 4.2-kb targeting vector consisting of IRES-LacZ with a neo-selectable marker was placed in between two BamHI sites found in the first exon of the gene locus. This vector replaced coding sequence for transmembrane domains 5 through 12. Northern blotting confirmed that the LacZ coding sequence was in a fusion transcript with the 5' portion of the gene's coding sequence. LacZ expression was detected in the thymic cortex and occasionally in the thymic corticomedullary junction. The N-terminal portion of the endogenous gene product, including transmembrane spans 1?4 of the protein, still remained expressed on the cell surface, as detected by flow cytometry. |
