| First Author | Tiwari VK | Year | 2012 |
| Journal | Proc Natl Acad Sci U S A | Volume | 109 |
| Issue | 16 | Pages | E934-43 |
| PubMed ID | 22474351 | Mgi Jnum | J:183621 |
| Mgi Id | MGI:5319012 | Doi | 10.1073/pnas.1119798109 |
| Citation | Tiwari VK, et al. (2012) Target genes of Topoisomerase IIbeta regulate neuronal survival and are defined by their chromatin state. Proc Natl Acad Sci U S A 109(16):E934-43 |
| abstractText | Topoisomerases are essential for DNA replication in dividing cells, but their genomic targets and function in postmitotic cells remain poorly understood. Here we show that a switch in the expression from Topoisomerases IIalpha (Top2alpha) to IIbeta (Top2beta) occurs during neuronal differentiation in vitro and in vivo. Genome-scale location analysis in stem cell-derived postmitotic neurons reveals Top2beta binding to chromosomal sites that are methylated at lysine 4 of histone H3, a feature of regulatory regions. Indeed Top2beta-bound sites are preferentially promoters and become targets during the transition from neuronal progenitors to neurons, at a time when cells exit the cell cycle. Absence of Top2beta protein or its activity leads to changes in transcription and chromatin accessibility at many target genes. Top2beta deficiency does not impair stem cell properties and early steps of neuronal differentiation but causes premature death of postmitotic neurons. This neuronal degeneration is caused by up-regulation of Ngfr p75, a gene bound and repressed by Top2beta. These findings suggest a chromatin-based targeting of Top2beta to regulatory regions in the genome to govern the transcriptional program associated with neuronal differentiation and longevity. |
