Primary Identifier | MGI:3522433 | Allele Type | Targeted |
Attribute String | Null/knockout, Reporter | Gene | Nkx6-2 |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A fragment of sequence containing the ORF in exons 1 and 2 was replaced with a lacZ-PGK-neo cassette. Northern blot of mutant brains indicated a lack of Nkx6-2 transcript, however, two signals were observed that hybridized to a lacZ probe. X-gal staining demonstrated lacZ was expressed prenatally in neurons and postnatally in oligodendrocytes of mutant embryos. |