| First Author | Pan J | Year | 2020 |
| Journal | J Neuroinflammation | Volume | 17 |
| Issue | 1 | Pages | 97 |
| PubMed ID | 32238175 | Mgi Jnum | J:352656 |
| Mgi Id | MGI:7704274 | Doi | 10.1186/s12974-020-01774-9 |
| Citation | Pan J, et al. (2020) Transcriptomic profiling of microglia and astrocytes throughout aging. J Neuroinflammation 17(1):97 |
| abstractText | BACKGROUND: Activation of microglia and astrocytes, a prominent hallmark of both aging and Alzheimer's disease (AD), has been suggested to contribute to aging and AD progression, but the underlying cellular and molecular mechanisms are largely unknown. METHODS: We performed RNA-seq analyses on microglia and astrocytes freshly isolated from wild-type and APP-PS1 (AD) mouse brains at five time points to elucidate their age-related gene-expression profiles. RESULTS: Our results showed that from 4 months onward, a set of age-related genes in microglia and astrocytes exhibited consistent upregulation or downregulation (termed "age-up"/"age-down" genes) relative to their expression at the young-adult stage (2 months). And most age-up genes were more highly expressed in AD mice at the same time points. Bioinformatic analyses revealed that the age-up genes in microglia were associated with the inflammatory response, whereas these genes in astrocytes included widely recognized AD risk genes, genes associated with synaptic transmission or elimination, and peptidase-inhibitor genes. CONCLUSIONS: Overall, our RNA-seq data provide a valuable resource for future investigations into the roles of microglia and astrocytes in aging- and amyloid-beta-induced AD pathologies. |
