| First Author | Bergoglio V | Year | 2004 |
| Journal | FEBS Lett | Volume | 566 |
| Issue | 1-3 | Pages | 147-50 |
| PubMed ID | 15147885 | Mgi Jnum | J:90141 |
| Mgi Id | MGI:3042621 | Doi | 10.1016/j.febslet.2004.04.020 |
| Citation | Bergoglio V, et al. (2004) Evidence of finely tuned expression of DNA polymerase beta in vivo using transgenic mice. FEBS Lett 566(1-3):147-150 |
| abstractText | DNA polymerase beta (Pol beta) is an error-prone repair DNA polymerase that has been shown to create genetic instability and tumorigenesis when overexpressed by only 2-fold in cells, suggesting that a rigorous regulation of its expression may be essential in vivo. To address this question, we have generated mice which express a transgene (Tg) bearing the Pol [Formula: see text] cDNA under the control of the ubiquitous promoter of the mouse H-2K gene from the major histocompatibility complex. These mice express the Tg only in thymus, an organ which normally contains the most abundant endogenous Pol [Formula: see text] mRNA and protein, supporting the idea of a tight regulation of Pol beta in vivo. Furthermore, we found no tumor incidence, suggesting that the single Pol beta overexpression event is not sufficient to initiate tumorigenesis in vivo. |
